Publications 2020-09-17T12:08:30+00:00



124– Francisco, V, Ferreira, L. “Effective conjugation of RNA molecules to nanocarriers for efficient and controlled delivery of RNA-based therapies”. (submitted)

123– Conniot, J, Talebian, S, Simoes, S, Ferreira, L*, Conde, J*. “Revisiting gene delivery to the brain: silencing versus editing”. (submitted)

122– Marta Esteves, Ricardo Abreu, Hugo Fernandes, Catarina Serra-Almeida, Ana ClaraCristóvão, Cláudia Saraiva, Raquel Ferreira, Lino Ferreira, Liliana Bernardino. “MicroRNA-124-enriched exosomes as a promising therapeutic approach for Parkinson’s disease”.(submitted)

121– Rai, A*, Ferreira, L. “Peptide decorated gold nanoparticles for biomedical applications”.(submitted).

120– Abreu, R, Ramos, C, Becher, C, Jesus, C, Martins, PC, Martins, PAT, Moreno, MJ,Fernandes, H * , Ferreira, L * . “Post-isolation enrichment of small extracellular vesicles with microRNAs”. (submitted).

119– Estronca, L, Francisco, V, Pitrez, P, Honorio, I, Carvalho, L, Blersch, J, Rai, A, Nissan, X, Simon, U, Graos, M, Saude, L, Ferreira, L. “Induced pluripotent stem cell-derived vascular networks to screen nano-bio interactions”. (submitted)



118– Bernardo, S, Donato, H, Ferreira, L, Freitas, JS. “Permeability of the blood brain barrier through the phases of ischemic stroke and relation with clinical outcome: protocol for a systematic review”. BMJ Open 2020 (in press).

117– Pitrez, PR, Estronca, L, Vazão, H, Harhouri, K, Thornton, D, Navarro, C, Egesipe, A-L,Lévy, N, Magalhães, JP, De Sandre-Giovannoli, A, Nissan, X, Rosell, A, Ferreira L. “A chip to study the vulnerability of progeroid smooth muscle cells to flow shear stress”. NatureCommunications 2020, 11, 4110.

116– Blersch, J, Francisco, V, Rebelo, C, Jiménez-Balsa, A, Antunes, H, Pinto, S, Simões, S,Rai, A, Ferreira, L. “A light-triggerable formulation to control the stability of pro-angiogenic transcription factor hypoxia inducible factor-1 (HIF-1)”. Nanoscale 2020, 12, 9935-9942.

115– Ferreira, R, Napoli, J, Enver, T, Bernardino, L*, Ferreira, L*. “Advances and challenges in retinoid delivery systems in therapeutic and regenerative medicine”. Nature Communications 2020, 11, 4265.

114– Abreu, R, Fernandes, H, Martins, PC, Sahoo, S, Emanueli, C, Ferreira, L. “Native and engineered extracellular vesicles for cardiovascular therapeutics”. Nature Reviews Cardiology 2020 (in press)

113– Katarzyna Buczak, Joanna M. Kirkpatrick, Felicia Trockenmueller, Deolinda Santinha,Lino Ferreira, Stephanie Roessler, Stephan Singer, Alessandro Ori, and Martin Beck. “Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry”. Nature Protocols 2020, 15, 2956-2979.

112– Blersch, J, Francisco, V, Rebelo, C, Jimenez, A, Henriques-Antunes, H, Gonzato, C,Pinto, S, Liedl, K, Haupt, K, Ferreira, L. “A light-activatable nanoparticle library for the controlled release of non-coding RNAs”. Angew Chem Int Ed 2020, 59(5), 1985-1991.


111– Yao, T-T, Wang, J, Yu, W-J, Gao, Q, Ferreira, L, Ren, K-F, Ji, J. “A photodynamic antibacterial spray-coating based on the host-guest immobilization of the photosensitizer methylene blue”. Journal of Materials Chemistry B 2019 (in press).

110– Chen, X-C, Huang, W-P, Ferreira, L, Ren, K-F, Ji, J. “Spatially Confining Surface Roughness on Exponentially Growing Polyelectrolyte Multilayer Films”. Advanced Materials Interfaces 2019 (in press).

109- Francisco, V, Lino, M, Ferreira, L. “A modular system for the delivery of small biomolecules based in supramolecular modified gold nanorods”. Journal of Nanobiotechnology 2019 (in press).

108- Ferreira, L. “What human blood-brain barrier models can tell us about BBB function and drug discovery?” Expert Opinion on Drug Discovery 2019 (in press)

107- Henriques-Antunes, H*, Cardoso, RMS,* Zonari, A, Correia, J, Leal, EC, Jimenez-Balsa, A, Lino, M, Barradas, A, Kostic, I, Gomes, C, Karp, JM, Carvalho, E, Ferreira, L. The kinetics of small extracellular vesicle delivery impacts skin tissue regeneration. ACS Nano 2019 (in press)

106- Praça, C, Rosa, S, Sevin, E, Cecchelli, R, Dehouck, M-P, Ferreira, L. “Derivation of brain-like endothelial cells from human pluripotent stem cell-derived endothelial progenitor cells”. Stem Cell Reports 2019 (in press)

105- Rosa, S, Pitrez, P, Fernandes, H*, Ferreira, L*. “A high-throughput screening method to identify compounds displaying human vascular embryonic toxicity”. Current protocols in Stem Cell Biology 201950(1), e93

104- Banerjee, A, Alves, V, Rondão, T, Sereno, J, Lino, M, Ribeiro, A, Abrunhosa, A*, Ferreira, L*. “A positron-emission tomography (PET)/magnetic resonance imaging (MRI) platform to track in vivo small extracellular vesicles”. Nanoscale 2019, 11, 13243-13248.

103– Rosa, S, Praça, C, Pitrez, PR, Gouveia, PJ, Aranguren, XL, Ricotti, L, Ferreira, L. “Functional characterization of iPSC-derived arterial- and venous-like endothelial cells”. Scientific Reports 2019, 9, 3826

102- Abecasis, B, Gomes-Alves, P, Rosa, S, Gouveia, P, Ferreira, L, Serra, M, Alves, PM. “A human induced pluripotent stem cell derived cardiac model: new inisghts in the crosstalk between cardiomyocytes and endothelial cells”. Biotechnology and Bioengineering 2019 (in press)

101- Querido, MM, Felgueiras HP, Rai, A, Monteiro, C, Oliveira, D, Ferreira, L, Martins MCL. “Antimicrobial coating to prevent infections associated with polyurethane without inducing platelet adhesion and activation”. Advanced Materials Interface 2019 (in press).

100- Ng, KS, Smith, JA, McAteer, MP, Mead, BE, Ware, J, Jackson, FO, Ferreira, L, Bure, K, Rowley, JA, Reeve, B, Brindley, DA, Karp, JM. “Bioprocess decision support tool for scalable manufacture of extracellular vesicles”. Biotechnology and Bioengineering 2019, 116(2), 307-319.


99- Gomes, AM, Kurochkin, I, Chang, B, Daniel, M, Law, K, Satija, N, Lachmann, A, Wang, Z, Ferreira, L, Ma´ayan, A, Chen, BK, Papatsenko, D, Lemischka, IR, Moore, KA, Pereira, C-F. “Coopertative transcription factor induction mediates hemogenic reprogramming”. Cell Reports 2018, 25(10), 2821-2835.

98- Ferreira, AF, Comune, M, Rai, A, Ferreira, L, Simões, PN. “Atomistic-level investigation of a LL37 conjugated gold nanoparticle by well-tempered metadynamics”. Journal of Physical Chemistry Part: B 2018, 122(35), 8359-8366.

97- Praça, C*, Rai, A*, Santos, T, Cristovão, AC, Pinho, SL, Cecchelli, R, Dehouck, M-P, Bernardino, L, Ferreira, L. “A nanoformulation for the preferential accumulation in adult neurogenic niches”. Journal Controlled Release 2018, 284, 57-72.

96- Lino MM, Simões S, Vilaça A, Antunes H, Zonari A, Ferreira L. “Modulation of angiogenic activity by light-activatable nanocarriers carrying miRNAs”. ACS Nano 2018, 12(6), 5207-5220.

95- Sargento-Freitas, João, Pereira, Anabela, Gomes, André, Freire, Paula, Matos, Teresa, Cardoso, Carla, Silva, Fernando, Cordeiro, Gustavo, Nunes, César, Galego, Orlando, Carda, José, Branco, João, Lourenço, Vítor, Cunha, Luís, Ferreira, Lino“STROKE34 study protocol: A randomized controlled phase IIa trial of intra-arterial CD34+ cells in acute ischemic stroke”. Frontiers Neurology 2018, 9, 302.

94-Patricia R. Pitrez, Luis Estronca, Helena Vazão, Anna-Laure Egesipe, Claire Navarro, Annachiara De Sandre-Giovannoli, Xavier Nissan, Lino Ferreira*. “Substrate topography modulates cell ageing in a Progeria model”. ACS Biomaterials Science and Engineering 2018, 4(5), 1498-1504.

93- Lima, A.F., May G., Colunga J., Pedreiro, S., Paiva, A., Ferreira, L., Enver T., Iborra F.J. , Pires das Neves, R.* “Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation”. Scientific Reports 2018, 8, 7210.

92- Jimenez-Balsa A, Pinto S, Quartin E, Lino MM, Francisco F, Ferreira L. “Light-activatable polymeric nanoparticles for intracellular delivery of proteins and small molecules”. Bioconjugate Chemistry 2018, 29 (5), pp 1485–1489.

91- Marta Machado-Pereira, Tiago Santos, Lino Ferreira, Liliana Bernardino, Raquel Ferreira. “Intravenous administration of retinoic acid-loaded polymeric nanoparticles prevents ischemic injury in the immature brain”. Neurosciences Letters2018, 673, 116-121.

90- Cláudia Saraiva, Daniela Talhada, Akhilesh Rai, Raquel Ferreira, Lino Ferreira, Tadeusz Wieloch, Liliana Bernardino*, Karsten Ruscher*.“MicroRNA-124-loaded nanoparticles protect and increase differentiation of neural stem cells in vitro but do not contribute to stroke outcome”. PLoS One2018, 13(3): e0193609.

89- Pinho S#, Macedo MH#, Rebelo C#, Sarmento B*, Ferreira L*. “Stem cells as vehicle and target of nanoparticles”. Drug Discovery Today 2018,23(5), 1071-1078.

88- Lino MM, Ferreira L. “Light-triggerable formulations for the intracellular controlled release of biomolecules”. Drug Discovery Today 2018,23(5), 1062-1070.

87- João Sargento-Freitas, Sezin Aday, César Nunes, Miguel Cordeiro, Ana Gouveia, Fernando Silva, Cristina Machado, Bruno Rodrigues, Gustavo Cordeiro, Carlos Ferreira, Miguel Castelo-Branco, Lino Ferreira* and Luís Cunha*. Endothelial progenitor cells influence acute and subacute stroke hemodynamics”. Journal of the Neurological Sciences 2018, 385, 119-125.

86- João Sargento-Freitas, Sezin Aday, César Nunes, Miguel Cordeiro, Ana Gouveia, Fernando Silva, Cristina Machado, Bruno Rodrigues, Gustavo Cordeiro, Carlos Ferreira, André Amorim, Susana Sousa, Ana Catarina Gomes, Miguel Castelo-Branco, Lino Ferreira* and Luís Cunha*. “Endothelial progenitor cells enhance subacute blood-brain barrier permeability in ischemic stroke”. Neurology 2018, 90(2), e127-e134.


85- Lino, M.M., Simões, S., Pinho, S., Ferreira, L. “Intracellular delivery of more than one protein with spatio-temporal control”. Nanoscale 2017, 9, 18668-18680.

84- Marta Machado-Pereira, Tiago Santos, Lino Ferreira, Liliana Bernardino, Raquel Ferreira. “Anti-inflammatory strategy for M2 microglial polarization using retinoic acid-loaded nanoparticles”.Mediators of Inflammation 2017, ID 6742427.

83- Comune M., Rai A., Chereddy K.K., Pinto S., Aday S., Ferreira A.F., Cunha R., Rodrigues R., Simões P.N., Preat V., Ferreira, L. “Antimicrobial peptide-nanoscale therapeutic formulation with high skin regenerative potential”. Journal Controlled Release 2017, 262, 58-71.

 82- Tiago Santos, Raquel Ferreira, Emanuel Quartin, Carlos Boto, Cláudia Saraiva, José Bragança, João Peça, Cecília Rodrigues, Lino Ferreira, Liliana Bernardino. “Light potentiates retinoic acid-induced neuronal differentiation of neural stem cells”. Acta Biomaterialia 2017, 59, 293-302.

81- Sezin Aday, Marie Besnier, Janet Zoldan, Laura Carreto, Jaimy Saif, Rui Fernandes, Tiago Santos, Liliana Bernardino, Robert Langer, Costanza Emanueli, Lino Ferreira. “Synthetic microparticles conjugated with VEGF165 improve the survival and function of endothelial progenitor cells via microRNA-17 inhibition”. Nature Communications 2017, 8, 747.

80- Teresa M. Ribeiro-Rodrigues, Tiago L. Laundos, Justin C Mason, Paulo Per, Rita Pereira-Carvalho, Ricardo Pereira, Vanessa Coelho-Santos, Ana Paula Silva, Rosa Fernandes, Mónica Zuzarte, Francisco J. Enguita, Marina C. Costa, Perpetua Pinto-do-Ó, Marta Teixeira Pinto, Pedro Gouveia, Lino Ferreira, Justin C Mason, Paulo Pereira, Brenda R Kwak, Diana S Nascimento, Henrique Girão. “Exosomes secreted by cardiomyocytes subjected to ischemia promote cardiac angiogenesis”. Cardiovascular Research 2017 (in press). Doi.org/10.1093/cvr/cvx118.

79- Gouveia P, Rosa S, Ricotti L, Abecassis B, Almeida HV, Monteiro L, Nunes J, Sofia Carvalho F, Serra M, Luckin S, Leonidovitch Kholkin A, Marques Alves P, Jorge Oliveira P, Carvalho R, Menciassi A, Neves RP, Ferreira LS. “Flexible nanofilms coated with aligned piezoelectric microfibers preserve the long-term contractility of cardiomyocytes”. Biomaterials 2017, 139, 213-228.

78- Luís Miguel Monteiro, Lino Ferreira, Perpétua Pinto-do-Ó, Diana S Nascimento. “Restoring Heart Function and Electrical Integrity: Closing the Circuit”. Regenerative Medicine 2017. doi:10.1038/s41536-017-0015-2.

77- Vazão H*, Rosa S*, Barata T, Costa R, Pereira P, Honório I, Papatsenko D, Benedito R, Saris D, Khademhosseini A, Pereira CF, Mercader N, Fernandes H, Ferreira L. High-throughput identification of small molecules that affect human embryonic vascular development”. PNAS 2017, 114(15), E3022-E3031. *Authors contributed equally.

76- Carlos Boto, Emanuel Quartin, Yijun Cai, Alberto Martin-Lorenzo, Maria Begona Garcia Cenador, Sandra Pinto, Rajeev Gupta, Tariq Enver, Isidro Sanchez-Garcia, Deng Li, Ricardo Neves, Lino Ferreira. “Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation”. Nature Communications 2017, 8:15204, DOI: 10.1038/ncomms15204.

75- Ribas, J., Zhang, Y.S., Pitrez, P.R., Leijten, J., Miscuglio, M., Rouwkema, J., Dokmeci, M., Nissan, X., Ferreira, L.*, Khademhosseini, A*. “Biomimetic strain exacerbates aging in a progeria-on-a-chip model”. Small 2017, 13, 1603737 *Authors contributed equally. DOI:10.1002/smll.201603737.


74– Lo Cícero, A., Jaskowiak, A.-L., Egesipe, A.-L-, Tournois, J., Brinon, B., Pitrez, P.R., Ferreira, L., Giovannoli, A. S., Levy, N., Nissan, X. “A high throughput phenotypic screening reveals compounds that counteract premature osteogenic differentiation of HGPS iPS-derived mesenchymal stem cells”. Scientific Reports 2016, 6:34798.

73– Cláudia Saraiva, Lino Ferreira, Liliana Bernardino. “Traceable microRNA-124 loaded nanoparticles as a new promising therapeutic tool for Parkinson’s disease”.  Neurogenesis 2016, 3 (1), e1256855

72– Machado-Pereira, M., Santos, T., Ferreira, L., Bernardino, L., Ferreira, R. “Challenging the great vascular wall: can we envision a simple yet comprehensive therapy for stroke?” J Tissue Engineering and Regenerative Medicine 2017, doi: 10.1002/term.2427.

71–  André Ferreira, Akhilesh Rai, Lino Ferreira, Pedro Simões. “Atomistic insights into the interaction of a cecropin-mellitin hybrid with a gold surface”.European biophysical journal 2016, DOI 10.1007/s00249-016-1160-z.

70– Cláudia M. Saraiva, José M. Paiva, Tiago Santos, Lino S. Ferreira, Liliana Bernardino. “MicroRNA-124 loaded nanoparticles enhance murine subventricular zone neurogenesis”.  J Controlled Release 2016, 235, 291-305.

69– Claudia Saraiva#, Catarina Almeida#, Raquel Ferreira, Tiago Santos, Lino Ferreira*, Liliana Bernardino*. Drug delivery into the brain: the blood brain barrier challenge and the nanomedicine solutions. J Controlled Release 2016, 235, 34-47. #,* Authors contributed equally.

68– Hajar Maleki*, Akhilesh Rai*, Sandra Pinto*, Marta Evangelista, António Portugal, Luísa Durães, Mohammad Imani, Abolreza Simchi, Lino Ferreira. “High antimicrobial activity and low human cell cytotoxicity of core-shell magnetic nanoparticles functionalized with an antimicrobial peptide”. ACS Applied Materials and Interfaces 2016, 8(18), 11366-78. *Authors contributed equally.

67– Ribas, J., Sadeghi, H., Manbachi, A., Leijten, J., Brinegar, K., Zhang, Y.S., Ferreira, L., Khademhosseini, A. “Cardiovascular organ-chip platforms for drug discovery and development”. Applied In Vitro Toxicology 2016, 2(2), 82-96.

66– Ferreira, R., Fonseca, M.C., Santos, T., Sargento-Freitas, J, Tjeng, R., Paiva, F., Castelo-Branco, M., Ferreira, L.S., Bernardino, L. “Retinoic acid-loaded polymeric nanoparticles enhance vascular regulation of neural stem cell survival and differentiation after ischaemia”. Nanoscale 2016, 8, 8126-8137.

65-Tiago Santos, Carlos Boto, Cláudia Saraiva, Liliana Bernardino*, Lino Ferreira*. Nanomedicine approaches to modulate stem cells in brain repair”. Trends in Biotechnology 2016, 34(6), 437-439. *Co-corresponding authors.

64-Akhilesh Rai, Sandra Pinto, Tiago Velho, Catarina Moita, André F. Ferreira, Urvish Trivedi, Marta Evangelista, Michela Comune, Kendra P. Rumbaugh, Pedro N. Simões, Luís Moita, Lino Ferreira. “One-pot synthesis of peptide-conjugated gold nanoparticles with in vivo antimicrobial efficacy in a systemic infection model”. Biomaterials 2016, 85, 99-110.

63-Aday, S., Cecchelli, R., Vanhuxeem, D., Dehouck, MP., Ferreira, L. ”Stem cell-based human blood brain barrier models for drug discovery and delivery”. Trends in Biotechnology 2016, 34(5), 382-393.

62-Rai, A, Pinto, S., Evangelista, M., Ferreira, L. “Antimicrobial peptide permanently immobilized on surfaces with high activity in the presence of serum and low cytotoxicity against human cells”. Acta Biomaterialia 2016, 3, 64-77.

61-Blondel, S., Egesipe, A.-L., Jaskowiak, A.-L., Tournois, J., Le Corf, A., Georges, P., Legraverend, M., Navarro, C., Pitrez, P.R., Ferreira, L., Bollot, G., Bauvais, C., Laustriat, D., De Sandre, Giovannoli, A., Levy, N., Peschanski, M., Nissan, X. “Pluripotent stem cells-based screening identifies aminopyrimidines as new modulators of prelamin A farnesylation for the treatment of Hutchinson Gilford progeria syndrome”. Cell Death and Differentiation 2016, 7:e2105.


60– Esteves, M., Saraiva, T., Rocha,S., Baltazar,G., Ferreira,L., Bernardino, L. “Retinoic acid-loaded polymeric nanoparticles induce neuroprotection in a mouse model of Parkinson’s disease.” Frontiers in Aging Neurosciences 2015, 7, 20.

59– Annachiara De Sandre-Giovannoli, MD, PhD; Rabah Ben Yaou, MD; France Leturcq; Carlos López-Otín; Xavier Nissan, PhD; Lino Ferreira, PhD; Catherine Coirault, MD, PHD; Catherine Badens, PhD; Nathalie Bonello-Palot; Claudia Cavadas, PhD; Antoine Muchir, PhD; Camilla Pellegrini, PhD; Karim Harhouri, PhD; Chiara Lanzuolo, PhD; Feriel Azibani, PhD; Brigitte Buendia, PhD; Pia Bernasconi; Patrice Roll, Md, PhD; Isaline Herrada; Sophie Zinn-Justin, PhD; Esma Ziat; Lorenzo Maggi, MD; Saida Ortolano, MD,PhD; Marie-Christine Vantyghem, MD, PhD; Faiza Bensmaine, MD; Michael Mitchell, PhD; Raoul CM Hennekam, MD, PhD; Susana Quijano-Roy, MD, PhD; Adele D’Amico, MD; Giuseppe Boriani, MD; Elena Biagini, MD; Karim Wahbi, MD, PhD; Denis Duboc, MD, PhD; Nicola Carboni, MD; Camille Vatier, MD, PhD; Corinne Vigouroux, MD, PhD; Tiziana E Mongini, MD; Alessandra Gambineri, MD; Christophe Béroud, PharmD, PhD; Gaëlle Blandin, PhD; Sara Benedetti, MD, PhD; David Araújo-Vilar, MD; Luigi Ravagnan; Ryszard Kole, PhD; Nicolas Levy, MD, PhD; Giovanna Lattanzi, PhD; Gisèle PhD Bonne, PhD Orphanet. An overview of new translational, clinical and therapeutic perspectives in laminopathies and other nuclear envelope-related diseases.  Proceedings of the 1st French-Italian meeting on laminopathies & other nuclear envelope-related diseases. Orphanet Journal of Rare Diseases 2015, 10(Suppl 2):O1.

 58– Pitrez, P., Rosa, S., Almeida, C., Nissam, X., Ferreira, L. “Vascular disease modeling using induced pluripotent stem cells: focus in Hutching-Gilford progeria syndrome”. Biochemical and Biophysical Research Communications 2015.

57– Ivana Kostic*, Isabel Fidalgo-Carvalho*, Sezin Aday, Helena Vazão, Tiago Carvalheiro, Mário Grãos, António Duarte, Carla Cardoso, Lino Gonçalves, Lina Carvalho, Artur Paiva, Lino Ferreira. “Lysophosphatidic acid enhances survival of human CD34+ cells in ischemic conditions mainly through peroxisome proliferator-activated receptor g.”Scientific Reports 2015,5:16406.


56- Chereddy, K.K., Her, C.-H., Moia, C., Comune, M., Vanacker, J., Zhu, H., Ferreira, L.S., Vandermeulen, G., Préat, V. “Antimicrobial peptide LL37-loaded PLGA nanoparticles promote wound healing activity”. J Control Release, 2014, 194, 138-147.

55- Aday S.*, Paiva J.*, Sousa S., Gomes R.S.M., Pedreiro S., So P.W., Carr C.A., Cochlin L., Gomes A.C., Paiva A., Ferreira L., Inflammatory modulation of hematopoietic stem cells by Magnetic Resonance Imaging (MRI)-detectable nanoparticles, RSC Advances, 2014, 4, 31706-31709. * Authors contributed equally.

54- Cechelli R.*, Aday S.*, Sevin E., Almeida C., Culot M., Dehouck L., Coisne C., Engelhardt B., Dehouck M.-P., Ferreira L., A stable and reproducible human blood-brain barrier model derived from stem cells, PLoS ONE, 2014, 9 (6), e99733. doi:10.1371/journal.pone.0099733. * Authors contributed equally.

53- Ricotti, L. Neves, R., Ciofani, G., Canale, C., Nitti, S., Mattoli, V., Mazzolai, B., Ferreira, L., Menciassi, A. “Boron nitride nanotube-mediated stimulation modulates F/G-actin ratio and mechanical properties of human dermal fibroblasts”. Journal of Nanoparticle Research 2014, 16(2), 1-14.

52- Santos, S.M., Peixoto, F., Ferreira, L., Jurado, A.S., Videira, R.A. ”Interaction of fullerene nanoparticles with biomembranes: from the partition in lipid membranes to effects on mitochondrial bioenergetics”. Toxicological Sciences 2014, 138(1), 117-129.

51– N. Lang*, M.J. Pereira*, I. Friehs, N. Vasilyev, E. N. Feins, K. Ablasser, E. O’Cearbhaill, C. Xu, A. Fabozzo, Y. Lee, R. Padera, S. Wasserman, F. Freudenthal, L.S. Ferreira, R. Langer, J.M. Karp, P. J. del Nido. “A biocompatible light-activated adhesive for minimally invasive repair of cardiovascular defects”. Science Translational Medicine 2014, 6(218), 218ra6. *Authors contributed equally.


50- Pereira, MJN, Sundback, CA, Lang, N, Cho, WK, Pomerantseva, I, Ouyang, B, Tao, SL, McHugh, K, Mwizerwa, O, Vemula, PK, Mochel, MC, Carter, DJ, Borenstein, JT, Langer, R, Ferreira, LS*, Karp, JM*, Masiakos, PT*. “Combined surface micropatterning and reactive chemistry maximizes tissue adhesion with minimal inflammation”. Advanced Health Materials 2013, doi: 10.1002/adhm.201300264. *Corresponding authors.

49- Lino, MM*, Paulo, CSO*, Vale, AC, Vaz, MF, Ferreira, LS. “Antifungal activity of dental resins containing amphotericin B-functionalized silica nanoparticles”. Dental Materials 2013, 29(10): e252-62. *Authors contributed equally.

48- Ricotti, L, Fujie, T, Vazão, H, Ciofani, G, Filippeschi, C, Corradini, I, Matteoli, M, Mattoli, V, Ferreira, L, Menciassi, A. “Boron nitride nanotube-mediated stimulation of cell co-culture on microengineered hydrogels.” PLoS One 2013, 8(8), e71707.

47– Paulo, CSO*, Lino, MM*, Matos, AA, Ferreira, LS. “Nanoparticles conjugated with amphotericin B are differentially taken up by human cells and after internalization increase the expression of heat shock protein”. Biomaterials 2013, 34(21), 5281-5293. *Authors contributed equally.

46- Gomes, RSM, Neves, R, Cochlin, L, Lima, AF, Carvalho, R, Korpisalo, P, Dragneva, G, Turunen, M, Liimatainen, T, Clarke, K, Yla-Herttuala, S, Carr, CA, Ferreira, L. “Nanoparticles for simultaneous cell tracking and pro-survival/angiogenic miRNA delivery in an ischemic animal model”. ACS Nano 2013, 7(4), 3362-3372.

45– Pereira, MJ, Oyuang, B, Sarkar, D, Lang, N, Sunbdback, C, Fiehs, I, Mureli, S, Pometantseva, I, McFaden, J, Sehn, JD, Mwizerwa, O, del Nido, P, Masiakos, P, Langer, R, Ferreira, LS*, Karp, JM*. “A highly tunable elastomer for local and controlled release of macromolecular therapeutics”. Advanced Materials 2013, 25(8), 1209-1215. *Authors contributed equally.


44– Santos, T, Ferreira, R, Maia, J, Agasse, F, Xapelli, S, Cortes, L, Bragança, J, Malva, JO, Ferreira, L*, Bernardino, L*. “Polymeric nanoparticles to control the differentiation of neural stem cells in the subventricular zone of the brain”. ACS Nano 2012, 6(12), 10463-10474. *Authors contributed equally.

43–  Maia*, J, Vazão*, H, Pedroso, DCS, Jesus, C, Brito, RMM, Gil, MH, Ferreira, L. “VEGF functionalized dextran has longer intracellular bioactivity than VEGF in endothelial cells”. Biomacromolecules 2012, 13, 2906-2916. *Authors contributed equally.

42–  Santos, T, Maia, J, Agasse, F, Xapelli, S, Ferreira, L, Bernardino, L. “Nanomedicine boosts neurogenesis: new strategies for brain repair”. Integrative Biology 2012, 4, 973-981.

41–  Ferreira, R, Santos, T, Gonçalves, J, Baltazar, G, Ferreira, L, Agasse, F, Malva, JO, Bernardino, L. “Histamine modulates microglial inflammatory response”. Journal of Neuroinflammation 2012, 9:90, doi: 10.1186/1742-2094-9-90.

40–  Botequim, D, Maia, J, Lino, MMF, Lopes, LMF, Simoes, PN, Ilharco, LM, Ferreira, L. “Nanoparticles and surfaces presenting antifungal, antibacterial and antiviral properties”. Langmuir 2012, 28(2), 7646-7656.

39–  Ricotti, L, Polini, A, Genchi, G, Ciofani, G, Iandolo, D, Vazão, H, Mattoli, V, Ferreira, L, Menciassi, A, Pisignano, D. “Proliferation and skeletal myotube formation capability of C2C12 and H9c2 cells on isotropic and anisotropic electrospun nanofibous PHB scaffolds”. Biomedical Devices 2012, 7(3), 035010.

38– Bernardino, L, Eiriz, MF, Santos, T, Xapelli, S, Grade, S, Rosa, A, Cortes, L, Ferreira, R, Bragança, J, Agasse, F, Ferreira, L, Malva, JO. “Histamine, a proneurogenic factor on the subventricular zone: a new approach for brain repair”. Stem Cells 2012, 30, 773-784.


37– Paulo, CSO, Pires das Neves, R, Ferreira, LS. “Nanoparticles for intracellular-targeted drug delivery”. Nanotechnology 2011, 22, 494002. *Authors contributed equally.

36– Kraehenbuehl, TP, Langer, R*, Ferreira, LS*. “Biomaterials for the self-renewal and differentiation of human pluripotent stem cells”. Nature Methods2011, 8(9), 731-736. *Corresponding authors.

35– Nunes-Pereira, MJ, Fidalgo-Carvalho, I, Karp, JM*, Ferreira, LS*. “Sensing the cardiac environment: exploiting cues for regeneration”. Journal of Cardiovascular Translational Research 2011, 4, 616-630. *Corresponding authors.

34– Vazão, H, Pires das Neves, R, Grâos, M, Ferreira, L. “Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells”. PLoS One 2011, 6(3): e17771.

33– Kraehenbuehl, T.P., Ferreira, L.S.*, Hayward, A.M., Nahrendorf, M., Vlies, A.J., Vasile, E., Weissleder, R., Langer, R.*, Hubbell, J.A.* “Human embryonic stem cell-derived microvascular grafts for cardiac tissue preservation after myocardial infarction”. Biomaterials 2011, 32(4), 1102-1109. *Corresponding authors.

32– Pedroso, DCS, Tellechea, A, Moura, L, Fidalgo-Carvalho, I, Duarte, J, Carvalho, E, Ferreira, L. “Improved survival, vascular differentiation and wound healing potential of umbilical cord stem cells co-cultured with endothelial cells”. PLoS One 2011, 6(1):e16114.

31– Maia, J,  Santos, T, Aday, S, Agasse, F, Cortes, L, Malva, JO, Bernardino, L, Ferreira, L. “Controlling the neuronal differentiation of stem cells by the intracellular delivery of retinoic acid-loaded nanoparticles. ACS Nano 2011, 5(1), 97-106. Authors contributed equally. L F and LB are corresponding authors.


30– Ferreira, L*,  Pedroso, DCS, Vazão, H, Gomes, RSM. “Stem cell-based therapies for heart regeneration: what did the bench teach us?” Cardiovascular & Haematological Disorders-Drug Targets 2010, 10, 173-185. *Corresponding author.

29– Paulo, C, Vidal, M, Ferreira, LS. ”Antifungal nanoparticles and surfaces”. Biomacromolecules 2010, 11, 2810-2817.

28– Levenberg, S*, Ferreira  LS*, Chen-Konak, L, Kraehenbuehl, TP, Langer, R. “Isolation, differentiation, and characterization of vascular cells derived from human embryonic stem cells”. Nature Protocols 2010, 5, 1115-1126. *Authors contributed equally.


27– Ferreira  LS. “Nanoparticles as tools to study and control stem cells”. Journal of Cellular Biochemistry 2009, 108(4), 746-752.

26– Ferreira  LS*, Zumbuehl A*. “Non-leaching surfaces capable of killing microorganisms on contact”. Journal of Materials Chemistry 2009, 19, 7796-7806. *Corresponding authors.

25– Kraehenbuehl TP, Ferreira LS, Zammaretti P, Hubbell JA, Langer R. “Cell-responsive hydrogel for encapsulation of vascular cells”. Biomaterials 2009, 30(26), 4318-4324.


24– Ferreira  L*, Karp JM, Nobre L, Langer R*. “New opportunities: the use of nanotechnologies to manipulate and track stem cells”. Cell Stem Cell 2008, 3(2), 136-146. *Corresponding authors.

23– Ferreira  L, Squier T, Park H, Choe H, Kohane DS, Langer R.“Human embryoid bodies containing nano- and micro-particulate delivery vehicles”. Advanced Materials 2008, 20, 2285-2291.

22– Mahdavi A*, Ferreira L*, Sundback C, Nichol JW, Chan EP, Carter JD,Bettinger C, Patanavanich S, Chignozha L, Ben-Joseph E, Galakatos A, Pryor H, Pomerantseva I, Masiakos P, Faquin W, Zumbuehl A, Hong S, Borenstein J, Vacanti J, Langer R, Karp JM. “A biodegradable and biocompatible Gecko-inspired tissue adhesive”.  Proceedings of the National Academy of Sciences 2008, 105(7), 2307-2312. *Authors contributed equally.

21– Fuller, J., Zugates, G., Ferreira, L., Ow, H., Nguyen, N., Wiesner, U., Langer, R. “Intercellular delivery of core-shell fluorescent silica nanoparticles”. Biomaterials 2008, 29, 1526-1532.


20– Zumbuehl, A.*, Ferreira, L.S.*, Kuhn, D., Astashkina, A., Long, L., Yeo, Y., Iaconis, T., Ghannoum, M., Fink, G.R., Langer, R., Kohane, D.S. “Antifungal hydrogels”. Proceedings of the National Academy of Sciences 2007, 104, 12994-12998. *Authors contributed equally.

19– Nijst, C., Joost, J., Karp, J.M., Ferreira, L., Zumbuehl, A., Bettinger, C., Langer, R. “Development of Multifunctional Photocurable Degradable Elastomers”. Biomacromolecules 2007, 8, 3067-3073.

18– Ferreira, L, Gerecht-Nir, S., Shieh, H.F., Watson, N., Rupnick, M, Dallabrida, S, M, Vunjak-Novakovic, G., Robert Langer, R. “Vascular progenitor cells isolated from human embryonic stem cells give rise to endothelial and smooth muscle-like cells and form vascular networks in vivo”. Circulation Research2007, 101, 286-294.

17– Gerecht-Nir, S., Burdick, J.A., Ferreira, L.S., Langer, R., Vunjak-Novakovic, G. “Propagation of undifferentiated human embryonic stem cells in 3-dimentional hyaluronic acid networks”. Proceedings of the National Academy of Sciences 2007, 104(27), 11298-11303.

16– Ferreira, L., Gerecht, S, Fuller, J, Shieh, HF, Vunjak-Novakovic, G, Langer, R. “Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells”. Biomaterials 2007, 28(17), 2706-2717.


15– Ferreira, L.*, Khademhosseini, A.*, Blumling, J, Yeh, J., Karp, J.M., Fukuda, J., Langer, R. “Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates”. Biomaterials 2006, 27, 5968-5977. *Authors contributed equally.

14– Jeffrey M. Karp, Lino S. Ferreira, Ali Khademhosseini, Albert H. Kwon, Judy Y. Yeh, Robert Langer. “Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro”. Stem cells 2006, 24(4), 835-843.

13– Ferreira, L., Figueiredo, M.M., Gil, M.H., Ramos, M.A. “Structural analysis of dextran-based hydrogels obtained chemoenzymatically”, Journal of Biomedical Materials Research: part B (Applied Biomaterials) 2006, 77B:55-64.


12– Maia, J., Ferreira, L., Carvalho, R., Ramos M.A., Gil, M.H. “Synthesis and characterization of new injectable and degradable dextran-based hydrogels.” Polymer 2005, 46, 9604-9614.

11– Ferreira, L., Evangelista M.B., Martins M.C.L., Esteves, J.L., Barbosa, M.A. “Improving the adhesion of poly(ethylene terephthalate) fibers to poly(hydroxyethyl methacrylate) hydrogels by ozone treatment: surface characterization and pull-out tests.” Polymer 2005, 46, 9840-9850.

10– Ferreira, L., Gil, M.H., Cabrita, A.M.S., Dordick, J.S. “Biocatalytic synthesis of highly ordered biocompatible and degradable dextran-based hydrogels”, Biomaterials 2005, 26, 4707-4716.


9– Ferreira, L., Rafael, A., Lamghari, M., Barbosa, M.A., Gil, M.H., Cabrita, A.M.S., Dordick, J.S. “Biocompatibility of  chemoenzymatically derived dextran hydrogels”, Journal of Biomedical Materials Research 2004, 68A, 584-596.


8– Ferreira, L., Ramos, M.A., Dordick, J.S., Gil, M.H. “Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (subtilisin Carlsberg)”, Journal of Molecular Catalysis B: Enzymatic 2003, 21, 189-199.


7– Ferreira, L., Ramos, M.A., Gil, M.H., Dordick, J.S. “Exquisite regioselectivity and transesterification activity enhancement of an immobilized Bacillus subtilis protease”. Biotechnology Progress 2002, 18(5), 986-993.

6– Ferreira, L., Kim, D.-Y., Carvalho, R., Geraldes, C.F.G.C., Gil, M.H., Dordick, J.S. “Biocatalytic polytransesterification of inulin with divinyladipate”. Chemistry of Materials 2002, 14(10), 4009-4011.

5– Ferreira, L., Gil M.H., Dordick, J.S. “Enzymatic synthesis of dextran-containing hydrogels”. Biomaterials  2002, 23, 3957-3967.

4– Ferreira, L., Carvalho, R., Gil, M.H., Dordick, J.S. “Enzymatic synthesis of inulin-containing hydrogels”. Biomacromolecules 2002, 3, 333-341.


3– Ferreira, L., Vidal, M.M., Gil, M.H. “Design of a drug-delivery system based on polyacrylamide hydrogels. Evaluation of structural properties”. Chem. Educator 2001, 6, 100-103.


2– Ferreira, L., Vidal, M.M., Gil, M.H. “Evaluation of poly(2-hydroxyethyl methacrylate) gels as drug delivery systems at different pH values”. International Journal of Pharmaceutics 2000, 194(2), 169-180.

1– Ferreira, L., Vidal, M.M., Geraldes, C.F.G.C., Gil, M.H. “Preparation and characterisation of gels based on sucrose modified with glycidyl methacrylate”. Carbohydrate Polymers 2000, 41(1), 15-24.


1– Gil M.H., Duarte, M.G., Ferreira, L., Geraldes, C.F.G. “Polymeric Biomaterials”. In: Polymer Chemistry, Eds: Melo, J.S.S., Moreno, M.J., Burrows, H.D., Gil, M.H. Coimbra University Press, Coimbra, 2004, p. 641-664.

2– Ferreira*, L., Khademhosseini*, A., Karp*, J.M., Gerecht-Nir*, S., Vunjak-Novakovic, G., Langer, R. “Embryonic stem cells as a cell source for tissue engineering”. In: “Principles of Tissue Engineering- 3rd edition”. Editors: Lanza, R., Langer, R., Vacanti, J.P. Elsevier/Academic Press, 2006. (*Authors contributed equally).

3– Luo, Y., Ferreira, L., Langer, R. “Three-dimensional scaffolds”. In: “Principles of Tissue Engineering- 3rd edition”. Editors: Lanza, R., Langer, R., Vacanti, J.P. Elsevier/Academic Press, 2006.

4– Karp, J. M., Mahdavi, A., Ferreira, L.S., Khademhosseini, A., Langer, R. “In vitro differentiation of human embryonic stem cells along osteogenic pathways”. In: “Human Embryonic Stem Cells: A Practical Handbook”, edited by: Sullivan S, Cowan C, Eggan K. John Wiley & Sons, Ltd., 2006.

5– Ferreira, L.*, Gerecht, S.*, Langer, R. “Vascular differentiation of human embryonic stem cells in bioactive hydrogel scaffolds”. In : “Human embryonic stem cells- methods and protocols”. Editors: Turksen, Walker. Humana Press 2008 (Authors contributed equally).

6– Spelke, D.P., Ortmann, D., Khademhosseini, A., Ferreira, L., Karp, J.M.”Method for EB formation- the microwell approach” In: “Embryonic stem cell therapy for osteodegenerative diseases”, Series Methods in Molecular Biology, Editor: Nieden, Nicole. Humana Press 2008.

7– Sarkar D, Zhao W, Schaefer S, Ankrum JA, Teo GS, Pereira MN, Ferreira L, Karp JM. “Overview of tissue engineering concepts and applications”, In: Biomaterials Science: an introduction  to materials in medicine”, 3rd edition, Editors: Ratner, BD, Hoffman, AS, Schoen, FJ, Lemons, JE. Academic Press 2009.

8– Kraehenbuehl TP, Aday S, Ferreira LS. “Scaffolding for three-dimensional embryonic vasculogenesis”, In: “Biophysical regulation of vascular differentiation and assembly”, Editor: Sharon Gerecht. Springer 2010.

9– Cho, WK, Pereira, MJMN, Lang, N, Lee, K, Mureli, S, Ferreira, L, Langer, RS, Karp, JM. “Gecko-inspired tape-based adhesives”, In: “Novel Biomaterials for wound closure”. Editor: Pan Stanford Publishing 2010.

10– Valente, AXCN*, Sousa, JAB, Outeiro, TF, Ferreira,L. “A stem-cell ageing hypothesis on the origin of Parkinson´s disease”. In “Science and Engineering in high-throughput biology including a theory on Parkinson´s disease. Editor: André X.C.N. Valente. Lulu 2011. *Corresponding author.

11– Vazão, H, Grãos, M, Ferreira, L. “Vascular differentiation of human pluripotent stem cells”. Editor: Hossein Baharvand, Springer, 2011.

12– Maia, J., Evangelista, M.B., Gil, M.H., Ferreira, L. “Dextran-based materials for biomedical applications”. Editor: Maria Helena Gil, Research Signpost, 2012.

13- Fernandes, H., Beijersbergen, R., Ferreira, L., Dechering, K., Moghe, P., Maniura-Weber, K. “Bioassay development”. In: “Materiomics: high-throughput screening of biomaterial properties”. Editors: Jan de Boer and Clemens A. Van Blitterswijk. Cambridge University Press, 2012.

14 Rai, A., Pinto, S., Paulo, C. Comune, M., Ferreira, L. Biocompatibility issues of organic and inorganic nanomaterials. Editor: Huijun Zhu, Bentham e-books, 2013.

15 Estronca, L., Ferreira, L. “Stem cells for the regeneration of chronic wounds: a translational perspective”. Editor: Joaquim Cabral, Elsevier, 2015.


1– Karp, J.M., Ferreira, L.S., Khademhosseini, A., Langer, R.S. “Amplification of specific cell populations from human embryonic stem cells”. WO2007022348, 2007 .

2– Ferreira, L.S., Gerecht-Nir, S., Vunjak-Novakovic, G., Langer, R.S. “Formation of vascular networks using human embryonic stem cells”. WO2007140340, 2007.

3– Nijst, C., Karp, J.M., Joost, J., Ferreira, L., Zumbuehl, A., Bettinger, C., Langer, R. “Development of Multifunctional Photocurable Degradable Elastomers”. WO 2007082304 and WO 2007082305, 2007.

4– Christopher Loose, William Shannan O´Shaughnessy, Lino Ferreira, Andreas Zumbuehl, Robert Langer, Gregory Stephanopolous. “Medical devices and coatings with non-leaching bioactive peptides”. WO 2007095393, 2007.

5– Zumbuehl, A., Ferreira, L.S., Kohane, D.S., Fink, G.R., Langer, R.S. “Coating of devices with gel, foam, film, particle or compn, comprising a polymer solvent and effector compounds, and their use for preventing infection and treatment of diseases. WO 2008005297.

6– Ferreira, L., Kohane, D., Langer, R.“Human embryoid bodies containing nano- and micro-particulate delivery vehicles“. WO 2009100128, 2009.

7– Lino Ferreira, Alborz Mahdavi, Cathryn Sundback, David J. D. Carter, Chris Bettinger, Andreas Zumbuehl, Jeff Borenstein, Joseph Vacanti,  Robert Langer, Jeffrey M. Karp. “A biodegradable and biocompatible Gecko-inspired tissue adhesive”. WO 2009067482, 2009.

8– Lino Ferreira, Cristiana Paulo. “ Antimicrobial nanoparticle conjugate”. Pending Patent, 2010.

9– Lino Ferreira, Dora Pedroso. “Method for improving the survival, vascular differentiation and wound healing potential of stem cells by co-culturing with endothelial cells in a biomimetic gel”. Pending Patent, 2010.

10– Maia, J, Santos, T, Malva, JO, Bernardino, L*, Ferreira, L*. “Nanoparticles to control stem cells”. Pending Patent, 2010.

11– Lino Ferreira, Dora Pedroso. “Composition and process to improve the therapeutic effect of stem cells”. PCT/IB2011/055342, 2011.

12– Lino Ferreira, Renata Gomes, Ricardo Neves. “Nanoparticles and uses thereof”. EPA 11398008.0, 2011.

13– Lino Ferreira, Isabel Carvalho. “Composition and method of stem cells for preservation of cardiac tissue”. PAT20121000032891.

14– Lino Ferreira, David Botequim, João Maia. “Antimicrobial complexes”. EP12398001, 2012.

15- Lino Ferreira, Akhilesh Rai “Antimicrobial coating compositions”. PAT20121000070064, 2012.

16- Lino Ferreira, Helena Vazão “Assessment of embryonic vascular toxicity by pluripotent stem cell-derived arterial endothelial cells on a microfluidic system”. PAT20121000095173, 2012.

17- Cecchelli, R, Aday, S, Sevin, E, Ferreira, L. “A human blood-brain barrier model derived from stem cells”. PAT20131000023338, 2013.

18- Boto, C., Neves, R., Ferreira, L. “Light-activatable polymeric nanoparticles”. PAT20131000066281, 2013.

19- Pitrez, P., Vazão, H., Ferreira, L. “Treatment of Hutchinson-Gilford Progeria Syndrome and diseases related to vascular ageing”. PAT20151000003657.

20- Comune, M., Ferreira, L. “A composition for use in the treatment of wound healing or in the treatment of ischemic aor vascular diseases”.        PAT20151000082124.

21- Lino, M., Ferreira, L. “A composition for controlled release of proteins”. PAT20151000082123.